An integrated metabolomic and proteomic approach for the identification of covalent inhibitors of the main protease (Mpro) of SARS-COV-2 from crude natural extractsArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 유전자 메커니즘, 치료제, [키워드] Analysis applied approach baicalein binding carried catalytic characteristic Chinese complementary complex Compound Covalent binder Cys145 database drug target electrophilic Final functional HIS163 HIS164 His41 independent inhibitor inhibitors Ion knowledge M pro M(pro) mass mass spectrometry maturation metabolomic metabolomics Michael molecular dynamics MPro nucleophilic peptide platform polyprotein protease protein-ligand Proteins proteomic proteomics residue SARS-CoV-2 SARS-CoV-2. Scutellaria baicalensis tested the SARS-CoV-2 [DOI] 10.1016/j.talanta.2022.123824 PMC 바로가기
Comparison of Wild Type DNA Sequence of Spike Protein from SARS-CoV-2 with Optimized Sequence on The Induction of Protective Responses Against SARS-Cov-2 Challenge in Mouse Model마우스 모델에서 SARS-CoV-2 공격에 대한 보호 반응 유도에 대한 최적화된 서열과 SARS-CoV-2의 스파이크 단백질 야생형 DNA 서열의 비교Article Published on 2022-12-312022-09-11 Journal: Human vaccines & immunotherapeutics [Category] COVID19(2023년), SARS, 비임상, 진단, [키워드] acid Against Antiviral binding breath CD4 CD8+ cellular immune responses cellular immunity challenge clinical trial contributing to COVID-19 CTL demonstrated DNA DNA vaccine expression full-length spike protein hACE2 humoral humoral and cellular immunity IFN-γ immune immune responses immunogen in vitro induction mice Model mouse Neutralizing antibodies optimization optimizations pGX-9501 platform Protective Protective effects protective response RBD binding response robust SARS-CoV-2 sequence spike Spike protein subsequent T cell T cells the vaccine Transgenic mouse Type virus wild-type wild-type sequence. [DOI] 10.1080/21645515.2021.2016201 PMC 바로가기 [Article Type] Article
Beyond COVID-19: Do biothermodynamic properties allow predicting the future evolution of SARS-CoV-2 variants?Research article Published on 2022-12-012022-10-05 Journal: Microbial Risk Analysis [Category] 변종, [키워드] Analysis binding biological property changes in COVID-19 pandemic driving Epidemiological study Evolution Evolution of SARS-CoV-2 Gibbs energy of binding Gibbs energy of growth growth Mechanistic model MERS-CoV Mutation new strain new virus Occurrence pandemic parameter performed predict risk SARS CoV-2 SARS-CoV statistical Strains the SARS-CoV-2 variants of SARS-CoV-2 Virus-host interaction viruses [DOI] 10.1016/j.mran.2022.100232 [Article Type] Research article
Synthesis of new Spiropyrazole derivatives under microwaves irradiation and docking study for inhibition the microbes and COVID-19Research article Published on 2022-12-012022-10-05 Journal: Journal of Molecular Structure [Category] 치료제, [키워드] 6LU7 addition analysis antimicrobial Antiviral binding Candida albican Complete Complexes COVID-19 derivative derivatives docking docking score docking study domain Effectiveness Hydrazonoyl halides indicated inhibit Interaction Laboratory microbe microwave Microwaves physicochemical property protease Seven Spiropyrazoles synthesis tested these compound with COVID-19 [DOI] 10.1016/j.molstruc.2022.133581 [Article Type] Research article
Pathogen-host adhesion between SARS-CoV-2 spike proteins from different variants and human ACE2 studied at single-molecule and single-cell levelsArticle Published on 2022-12-012022-11-16 Journal: Emerging Microbes & Infections [Category] SARS, 변종, 유전자 메커니즘, [키워드] ACE2 ACE2-RBD acute respiratory syndrome addition adhere binding complex coronavirus Delta delta variant destabilized effective force spectroscopy highest host cell human ACE2 human Angiotensin-converting enzyme immunization Infection inhibit Interaction investigated molecular dynamics molecular mechanism monoclonal antibody Mutation mutations N501Y Neutralizing neutralizing antibody omicron pathogen-host adhesion. Probability produced RBD Receptor binding domain S477N SARS-CoV-2 SARS-CoV-2 spike protein single-cell single-cell level Spike protein tested the spike protein variant variants virus wild type [DOI] 10.1080/22221751.2022.2128887 PMC 바로가기
Omicron BA.1 and BA.2 variants increase the interactions of SARS-CoV-2 spike glycoprotein with ACE2Article Published on 2022-12-012022-11-16 Journal: Journal of molecular graphics & modelling [Category] COVID19(2023년), SARS, 변종, 치료기술, [키워드] ACE2 ACE2 receptor Affect angiotensin-converting enzyme 2 Area B.1.1.529 binding binding efficiency binding free energy conformation domain dominant explicit full-length glycans glycoprotein host cells hydrophobic interaction infect Infection initiated Interaction interactions ions Mechanics MMPBSA molecular dynamics Molecular dynamics simulation molecular dynamics simulations mutated Mutation omicron Omicron variant performed RBD RBD-ACE2 receptor residue Salt Bridge SARS-CoV-2 SARS-CoV-2 spike glycoprotein Simulation spike spike glycoprotein spike glycoprotein. strain surface the receptor-binding domain trajectory variant variant of SARS-CoV-2 variants wild-type [DOI] 10.1016/j.jmgm.2022.108286 PMC 바로가기
Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRVSARS-CoV-2 단백질 소단위 백신 ZF2001 및 mRNA 백신 RRV의 타고난 면역 특징의 전사체 분석Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 유전자 메커니즘, 진단, [키워드] Activation Analysis Anti-viral antibody approach binding CD74 Cell comparable COVID-19 cytokine Cytokines early response elicited expression level expression of gene framework Gene Expression Genes immune response immune responses immunization innate immune innate immune response Innate immunity involved mapped MHC class mRNA mRNA vaccine neutralization Neutralization antibody Pattern recognition pattern recognition receptor Pattern recognition receptors post vaccination Protein subunit protein subunit vaccine PRRs RNA degradation robust SARS-CoV-2 SARS-CoV-2 protein subunit Subunit vaccine Tap1 titre titres Transcription transcriptional Transcriptome transcriptome sequencing Transcriptome sequencing. transcriptomic transduction Vaccine ZF2001 [DOI] 10.1080/22221751.2022.2059404 PMC 바로가기 [Article Type] Article
The prominent role of a CDR1 somatic hypermutation for convergent IGHV3-53/3-66 antibodies in binding to SARS-CoV-2SARS-CoV-2에 결합하는 수렴형 IGHV3-53/3-66 항체에 대한 CDR1 체세포 과돌연변이의 두드러진 역할Article Published on 2022-12-012022-09-12 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 진단, [키워드] acute respiratory syndrome Analysis antibodies antibody antibody affinity binding binding affinity Cdr1 contributed Coronavirus-2 COVID-19 Frequency functional germline gene Humoral immunity IGHV3-53/3-66 knowledge mAb mAbs monoclonal monoclonal antibody Mutation other coronaviruses SARS-CoV-2 somatic hypermutation. somatic hypermutations Treatment [DOI] 10.1080/22221751.2022.2063074 PMC 바로가기 [Article Type] Article
Evaluation of humoral immune responses induced by different SARS-CoV-2 spike trimers from wild-type and emerging variants with individual, sequential, and combinational delivered strategiesArticle Published on 2022-12-012022-11-15 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome Antibody Response antibody response against autologous binding circulating coronavirus coronavirus disease COVID-19 Delta effective heterogenous homologous humoral immune response immunization immunogenicity indicated induce monovalent vaccine multivalent vaccine neutralizing antibody Neutralizing antibody response omicron produced regimen SARS-CoV-2 SARS-CoV-2 spike trimer significantly spike Spike protein spike trimer strain supported trimer Vaccination strategies Vaccination strategy Vaccine Vaccines variant Variant. variants viruses wild type wild-type [DOI] 10.1002/jmv.28081 PMC 바로가기
ACE2 decoy receptor generated by high-throughput saturation mutagenesis efficiently neutralizes SARS-CoV-2 and its prevalent variants고처리량 포화 돌연변이 유발에 의해 생성된 ACE2 미끼 수용체는 SARS-CoV-2 및 그 유행하는 변이체를 효율적으로 중화합니다Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 ACE2 binding ACE2-RBD binding approach binding binding affinity C-terminal cellular receptor changes in convalescent sera Critical critical point D614G decoy receptor domain Effect fused global pandemic glycosylation site High-throughput screening increase in Microarray monoclonal antibodies Mutagenesis mutations neutralization neutralize neutralized Omicron variant Point mutation prevalent protease Protein protein engineering. Pseudotyped virus RBD RBD binding RBD-ACE2 receptor Receptor binding domain rescued SARS-CoV-2 SARS-CoV-2 virus significantly single-cell soluble ACE2 Spike protein the SARS-CoV-2 the SARS-CoV-2 virus therapeutic potential Treatment Vaccine variant variants viral entry viral spike protein wild-type [DOI] 10.1080/22221751.2022.2079426 PMC 바로가기 [Article Type] Article